Search results for "Membrane protein complex"
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GreenCut proteinCPLD49 ofChlamydomonas reinhardtiiassociates with thylakoid membranes and is required for cytochromeb6fcomplex accumulation
2018
The GreenCut encompasses a suite of nucleus-encoded proteins with orthologs among green lineage organisms (plants, green algae), but that are absent or poorly conserved in non-photosynthetic/heterotrophic organisms. In Chlamydomonas reinhardtii, CPLD49 (Conserved in Plant Lineage and Diatoms49) is an uncharacterized GreenCut protein that is critical for maintaining normal photosynthetic function. We demonstrate that a cpld49 mutant has impaired photoautotrophic growth under high-light conditions. The mutant exhibits a nearly 90% reduction in the level of the cytochrome b6 f complex (Cytb6 f), which impacts linear and cyclic electron transport, but does not compromise the ability of the stra…
111 Bioluminescence analysis and numerical evaluation of ATP-synthesis by native and reconstituted membranes containing bacterial ATP-synthase
1992
ATP-synthase is a large membrane protein complex, which plays a key role in the energy metabolism of most organisms. It consists of at least eight types of subunits and can be isolated and purified from several organisms, e.g. bacteria. The enzyme couples two reversible reactions: vectorial proton transport through a membrane and synthesis of the energy rich molecule ATP. Both can be investigated with vesicles from native membranes or with reconstituted liposomes from purified ATPsynthase. The analysis is complicated because ATP-synthase catalyzes ATP-synthesis as well as ATP-hydrolysis. Furthermore the ATP level of membrane samples is influenced by adenylate kinase activities of other enzy…